The potential is also very high for the manufacture of highly efficacious drugs for the treatment of sleeping sickness and/or cancer through synthetic modification of isolated bioactive phytoconstituents from this plant. Ethno-botanical survey and preliminary evaluation of some selected medicinal plants used in the treatment of tuberculosis in some parts of Northern Nigeria. Ethno-botanical survey and preliminary This is because of the strong link between Chemotherapy of human African trypanosomiasis (sleeping sickness) and cancer chemotherapy as every drug currently used for the treatment of sleeping sickness has some form of anticancer activity. Oladosu OP (2012b). Methods: ESBLs-producing E. coli and Klebsiella spp were isolated from clinical and environmental specimens and incubated with Acacia nilotica pod lysates. Two of the test bacteria were susceptible to the ethyl acetate extract, while ten and seven were susceptible to the acetone and chloroform extracts respectively at the test concentration of 5 mg/ml. Industrial microorganisms are those microorganisms which have been selected carefully to make one or more specific products. Based on the concept of description level described in the previous chapter, it analyzes key hypotheses such as the homogeneity of a system. Sci. PhD Thesis, University of Ibadan. brucei infection in mice, while doses of 50, 100, 200, 300, and 400mgkg-1 body weight per day of the partially purified extract completely cured the experimental infection in mice within two days. UWO (University of Western Ontario Culture Collection, Department of Plant Sciences), Ontario N6A 587, Canada. What is the reserve food material in red algae? Purpose: To investigate lysates from Acacia nilotica pods for their antimicrobial and cytotoxic activities against a variety of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as well as methicillin resistant Staphylococci aureus (MRSA). Difference between Old Biotechnology and Modern Biotechnology, Microbial Diseases: Top 27 Things to Know About| Microbiology. 2, Time Kill-kinetics Antibacterial Study of Acacia nilotica, Table 3. Moreover, the plant is used in folk medicine for the treatment of cancer. FPLC revealed eight peaks, with three of them representing compounds that had maximum bactericidal activity against all the tested isolates, but showed < 30 % host cell cytotoxicity. Privacy Policy3. The addition of new genetic engineering techniques has brightened the chances for yet greater yield. Part 1. What are the different sources of air pollution? (credit right: modification of work by Centers for Disease Control and Prevention), In fact, this chapter attempts to find the general theoretical bases of the original approach of polyphasic dispersed systems (PDS). 17 Applications of Microbiology 1. medicinal plants used in the traditional treatment of tuberculosis for bioactive compounds IMI (International Mycological Institute) Bakeham Lane, Egham, Surrey TW 209 TY, UK. methanolic extraction was done so as to extract both polar and non polar components of the plants. Bark powder of Acacia nilotica was successively extracted with petroleum ether, methanol and distilled water. Industrial microbiology came into existence initially by establishment of alcoholic fermentation processes to produce wine and beer. CMI (Commonwealth Mycological Institute presently known as International Mycological Institute) Kew, UK. (With Methods)| Industrial Microbiology, How is Cheese Made Step by Step: Principles, Production and Process, Enzyme Production and Purification: Extraction & Separation Methods | Industrial Microbiology, Fermentation of Olives: Process, Control, Problems, Abnormalities and Developments, The best answers are voted up and rise to the top. The potential toxicity of Acacia nilotica was investigated in rats maintained on 2% and 8% acacia diet for 2 and 4 weeks. Content Guidelines 2. Yeast: Origin, Reproduction, Life Cycle and Growth Requirements | Industrial Microbiology, How is Bread Made Step by Step? 19 mm diameter spectrophotometer tubes until absorbance of 0.1 was reached and a plotted standard, for assessment of antimicrobial activity using time-kill ki, respectively. Sci. In vitro bioactivity of crude extracts of Lippia javanica on clinical isolates of Helicobacter pylori: Preliminary phytochemical screening. The post-antibiotic effects of gentamicin and ciprofloxacin at 1x, 2x and 4x MIC on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 were studied using a spectrophotometric method and the classic method of viable counts on agar as a reference. (2) Microbial technology with genetically engineered microorganisms in which new genes have been inserted. Acacia nilotica Willd. The objective of this study was to evaluate the therapeutic effects of Acacia nilotica in experimental Trypanosoma brucei brucei infection in mice. Egyptian thorn, red thorn, Babool, babul in India [1]. All test bacteria were susceptible to the methanol extract, while none was susceptible to the aqueous extract. Thus, almost 50,000 times increase was possible by mutation and selection without involving genetic engineering techniques. Why does plant cell possess large sized vacuole? It does not deal with biotechnology or microbiology in particular. Share Your PPT File. Basic Techniques. Applications of Microbiology Microbiology is one of the most applied branches of science. CBS (Central Bureau Voor Schimmelcultures) Oousterstraat 1, Baarn, The Netherlands. This website includes study notes, research papers, essays, articles and other allied information submitted by visitors like YOU. Figure 1. Synthesis of {2-[3-(2-ammonioethoxy)benzoyl]ethyl}trimethylammonium bromide hydrobromide for the aff... ChemInform Abstract: Genetic Engineering. Therapeutic evaluation of Acacia nilotica (Linn) stem bark extract in experimental African trypanosomiasis, Antibacterial and Cytotoxic Activities of Acacia nilotica Lam (Mimosaceae) Methanol Extracts Against Extended Spectrum Beta-Lactamase Producing Escherichia coli and Klebsiella Species, In vitro antibacterial time kill studies of leaves extracts of Helichrysum longifolium, Antidiarrhoeal activity of Acacia nilotica Willd. Phytochemical analysis showed the presence of carbohydrates, saponin, tannin and cardiac glycoside. CDDA (Canadian Department of Agriculture) Ottawa, Canada. Time-kill kinetics antibacterial study of extract of. In this chapter, we will explore some of those tools, especially as they relate to applications in medicine and health care. 2. Disclaimer Copyright, Share Your Knowledge This extract showed action against castor oil, magnesium sulphate induced diarrhoea and enteropooling activity due to castor oil treatment as well as on normal as well as barium chloride induced peristalsis of small intestine significantly in mice, so also antimicrobial activity against common pathogens responsible for diarrhoea in vitro. Other molecular tools, such as restriction enzymes and plasmids obtained from microorganisms, allow scientists to insert genes from humans or other organisms into microorganisms. pp 903. It critically reconsiders the most common assumptions directly, Join ResearchGate to discover and stay up-to-date with the latest research from leading experts in, Access scientific knowledge from anywhere. These are just a few of the numerous applications of microbial genetics that we will explore in this chapter. Exploratory studies revealed presence of significant antidiarrhoeal activity only to methanol extract of Acacia nilotica (AN). IMY (Institute of Microbiology & Virology) Academy of Sciences of the Ukranian S.S.R., Kiev. FAT (Faculty of Agriculture, Tokyo University) Tokyo, Japan. Some of the general culture collections with their abbreviation, full name and locations are given below: AMRC (FAO-WHO International Reference Centre for Animal Mycoplasma) Institute of Medical Microbiology, University of Aarhas, Denmark. At higher con-, Theory and Applications of Microbiology and Biotechnology Vol. used in physical chemistry systems and, in particular, the usual ways of theoretical representation, such as mathematical modeling, for example. Time-kill kinetics antibacterial study of A. nilotica against P. aeruginosa, Time-kill kinetics antibacterial study of extract of A. nilotica against E. coli, All figure content in this area was uploaded by Peters Oluwale Oladosu, All content in this area was uploaded by Peters Oluwale Oladosu on Dec 21, 2019, Print ISBN: 978-93-89562-78-1, eBook ISBN: 978-93-89562-79-8, analyzed by percentage and log reduction. The modification is done to achieve a target of higher yield. The bactericidal activity and cytotoxic effects of the lysates were evaluated while fast protein liquid chromatography (size exclusion FPLC) was used to identify the various compounds showing bactericidal activity. This chapter is the most general of this book. Microbial biotechnology can be divided under two subheadings: (1) Traditional microbial technology which is the large scale manufacture of products which are normally produced by microorganisms.